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In vivo and in vitro evidence for epidermal H 2 O 2 ‐mediated oxidative stress in piebaldism
Author(s) -
Vafaee Tayyebeh,
Rokos Hartmut,
Salem Mohamed M. A. E. L.,
Schallreuter Karin U.
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.00966.x
Subject(s) - methionine sulfoxide reductase , thioredoxin reductase , oxidative stress , msra , vitiligo , in vivo , methionine , biochemistry , superoxide dismutase , pteridine , biology , chemistry , thioredoxin , enzyme , immunology , genetics , amino acid
Please cite this paper as:In vivo and in vitro evidence for epidermal H 2 O 2 ‐mediated oxidative stress in piebaldism. Experimental Dermatology 2010; 19 : 883–887. Abstract: Piebaldism is characterised by the absence of pigment in patches on the skin, usually present at birth. Mutations in the kit gene are documented. Clinically this disorder can mimic vitiligo. Here, we show for the first time the presence of oxidised pteridine‐induced fluorescence in association with H 2 O 2 ‐mediated stress in piebald patches employing Wood’s light and in vivo FT‐Raman spectroscopy. In situ immunofluorescence data revealed low catalase and methionine sulphoxide reductase A (MSRA) levels whereas thioredoxin reductase and methionine sulphoxide reductase B (MSRB) are not affected. We also show low superoxide dismutase levels in these patients. The presence of thioredoxin reductase provides capacity to reduce H 2 O 2 , a mechanism which is absent in vitiligo. Importantly, this enzyme reduces biopterin back to the functioning cofactor 6‐tetrahydrobiopterin. The absence of MSRA indicates deficient methionine sulphoxide repair in the cytosol, meanwhile the presence of MSRB is helpful to protect the nucleus. Taken together, we have identified H 2 O 2 ‐mediated stress in piebald skin with distinct differences to vitiligo.