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Anti‐IL5 decreases the number of eosinophils but not the severity of dermatitis in Sharpin‐deficient mice
Author(s) -
Renninger Matthew L.,
Seymour Rosemarie E.,
Whiteley Laurence O.,
Sundberg John P.,
HogenEsch Harm
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.00944.x
Subject(s) - eosinophil , atopic dermatitis , immunology , biology , asthma
Please cite this paper as: Anti‐IL5 decreases the number of eosinophils but not the severity of dermatitis in Sharpin‐deficient mice. Experimental Dermatology 2010; 19: 252–258. Abstract: Sharpin‐deficient ( Sharpin cpdm ) mutant mice develop a chronic eosinophilic dermatitis. To determine the efficacy of eosinophil‐depletion in chronic inflammation, Sharpin cpdm mice were treated with anti‐IL5 antibodies. Mice treated with anti‐IL5 had a 90% reduction of circulating eosinophils and a 50% decrease in cutaneous eosinophils after 10 days compared with sham‐treated littermates. Reducing the number of eosinophils resulted in increased severity of alopecia and erythema and a significant increase in epidermal thickness. Skin homogenates from mice treated with anti‐IL5 had decreased mRNA expression of arylsulfatase B ( Arsb ), diamine oxidase (amiloride‐binding protein 1, also called histaminase; Abp1 ) and Il10 , which are mediators that eosinophils may release to quench inflammation. Skin homogenates from mice treated with anti‐IL5 also had decreased mRNA expression of Il4 , Il5 , Ccl11 , kit ligand ( Kitl ) and Tgfa ; and increased mRNA expression of Tgfb1 , Mmp12 and tenascin C ( Tnc ). In order to further decrease the accumulation of eosinophils, Sharpin cpdm mice were crossed with IL5 null mice. Il5 −/− , Sharpin cpdm / Sharpin cpdm mice had a 98% reduction of circulating eosinophils and a 95% decrease in cutaneous eosinophils compared with IL5‐sufficient Sharpin cpdm mice. The severity of the lesions was similar between IL5‐sufficient and IL5‐deficient mice. Double mutant mice had a significant decrease in Abp1 , and a significant increase in Tgfb1 , Mmp12 and Tnc mRNA compared with controls. These data indicate that eosinophils are not essential for the development of dermatitis in Sharpin cpdm mice and suggest that eosinophils have both pro‐inflammatory and anti‐inflammatory roles in the skin of these mice.