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MAP17 is associated with the T‐helper cell cytokine‐induced down‐regulation of filaggrin transcription in human keratinocytes
Author(s) -
Noh Minsoo,
Yeo Hyeonju,
Ko Jaeyoung,
Kim Han Kon,
Lee ChangHoon
Publication year - 2010
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.00902.x
Subject(s) - loricrin , filaggrin , involucrin , biology , hacat , thymic stromal lymphopoietin , transcription factor , cytokine , keratinocyte , immunology , cellular differentiation , microbiology and biotechnology , gene , genetics , atopic dermatitis , cell culture
Please cite this paper as: MAP17 is associated with the T‐helper cell cytokine‐induced down‐regulation of filaggrin transcription in human keratinocytes. Experimental Dermatology 2009. Abstract: In the meta‐analysis of public microarray databases for different skin diseases, we revealed seven commonly up‐regulated genes, DSG3 , KRT6 , MAP17 , PLSCR1 , RPM2 , SOD2 and SPRR2B . We postulated that the genes selected from the meta‐analysis may be potentially associated with the abnormal keratinocyte differentiation. To demonstrate this postulation, we alternatively evaluated whether the genes of interest in the meta‐analysis can be regulated by T‐helper (Th) cell cytokines in normal human epidermal keratinocytes (NHEK). We found that MAP17 was significantly up‐regulated in response to interferon‐γ, interleukin 4 (IL‐4), IL‐6, IL‐17A or IL‐22 in NHEK. Interestingly, MAP17 was originally reported to interact with PDZK1; in turn, the PDZK1 gene is localized within the atopic dermatitis‐linked region on human chromosome 1q21. In an attempt to evaluate whether MAP17 regulates the expression of cornified envelope‐associated genes at the 1q21 locus, such as filaggrin, loricrin and involucrin, we found that the over‐expression of MAP17 in HaCaT keratinocytes significantly decreased the expression of filaggrin. Taken together, the Th cell cytokine‐induced up‐regulation of MAP17 expression may be linked to the down‐regulation of filaggrin in NHEK, which may be associated with the abnormal epidermal differentiation observed in the dermatological diseases.