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The effect of moesin overexpression on ageing of human dermal microvascular endothelial cells
Author(s) -
Lee Ju Hee,
Hong In Ae,
Oh Sang Ho,
Kwon Yeon Sook,
Cho Soo Hyun,
Lee Kwang Hoon
Publication year - 2009
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.00898.x
Subject(s) - moesin , ageing , senescence , microbiology and biotechnology , endothelial stem cell , endothelium , mechanism (biology) , cyclin d1 , biology , cell , cell cycle , cytoskeleton , in vitro , biochemistry , genetics , ezrin , philosophy , epistemology
Senescence of microvascular endothelial cells is known to play an important role in the pathophysiology of vascular diseases related to ageing, but the accurate mechanism or related genes are not known. Moesin, a cytoskeletal protein and the most potent candidate as an ageing‐related protein, showed obvious changes in expression when compared before and after ageing. In this study, a lentivirus was used to overexpress moesin in endothelial cells. The expression of cell cycle mediators such as p16, cyclin D1 and cdk4, which can be the markers of ageing, was compared by RNA and was shown to be suppressed in moesin overexpressed endothelial cells. In conclusion, it can be said that the expression of moesin delays senescence of human dermal microvascular endothelial cells and this fundamental discovery can be used as a basis for understanding the mechanism of ageing and age‐related diseases.