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A guide to hair follicle analysis by transmission electron microscopy: technique and practice
Author(s) -
Morioka Kiyokazu
Publication year - 2009
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.00879.x
Subject(s) - keratin , hair follicle , organelle , electron microscope , microbiology and biotechnology , biology , transmission electron microscopy , pathology , cell , medicine , materials science , nanotechnology , biochemistry , optics , physics
Hair follicles contain several tissues and cell types that differentiate down distinct pathways to provide for growth, keratinization and the maintenance of the hair shaft. Electron microscopy is useful for examining the morphological characteristics of developing hair follicles, including special types of keratinization, the timing of keratinization, programmed cell death, cell adhesion and separation, cell movement and changes in organelles. Hair follicles are one of the more challenging targets for electron microscopic analysis, and the use of neonatal animals combined with careful treatment of the samples can yield informative photomicrographs. Detailed protocols and examples of a number of techniques are presented here.