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Murine mast cells secrete a unique profile of cytokines and prostaglandins in response to distinct TLR2 ligands
Author(s) -
MrabetDahbi Salima,
Metz Martin,
Dudeck Anne,
Zuberbier Torsten,
Maurer Marcus
Publication year - 2009
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2009.00878.x
Subject(s) - tlr2 , lipoteichoic acid , microbiology and biotechnology , cytokine , receptor , secretion , biology , prostaglandin d2 , immunology , chemistry , signal transduction , tlr4 , biochemistry , staphylococcus aureus , bacteria , genetics
Mast cells (MCs) are important effector cells in host defense against bacteria. In the course of a bacterial infection, MCs can be activated by various mechanisms, i.e. bacterial toxins, endogenously produced infection‐associated peptides or via complement receptors, fimbrial adhesion molecules and toll‐like receptors (TLRs). While some of these mechanisms are well established, the effects of TLR2 ligand‐driven MC activation are far less understood. Here, we show that murine mature connective tissue‐type MCs, but not immature bone marrow‐derived cultured mast cells, express significant amounts of full length TLR2 on their surface. Activation by various TLR2 ligands only induces the selective release of cytokines in peritoneum‐derived cultured mast cells (PCMCs) with preferential secretion of pro‐inflammatory cytokines (IL‐6 > IL‐17 > IFN‐γ TNF > IL‐1 > GM‐CSF) upon stimulation with lipoteichoic acid (LTA). This response is much lower in PCMCs stimulated with the TLR2/6 agonist macrophage‐activating lipopeptide‐2 (MALP‐2), which most prominently triggers the release of the immunomodulatory cytokine IL‐10. Furthermore, only LTA but not MALP‐2 induces prostaglandin D2 secretion which is again restricted to the mature MC phenotype. These findings suggest that TLR2 ligand‐mediated activation of mature MCs, i.e. tissue‐residing cells, which most likely occurs during infection, can selectively raise a potent inflammatory or anti‐inflammatory response, depending on TLRs which are engaged.