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Protein kinase C isoenzymes differentially regulate the differentiation‐dependent expression of adhesion molecules in human epidermal keratinocytes
Author(s) -
Szegedi Andrea,
Páyer Edit,
Czifra Gabriella,
Tóth Balázs I.,
Schmidt Emese,
Kovács László,
Blumberg Peter M.,
Bíró Tamás
Publication year - 2009
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2008.00771.x
Subject(s) - hacat , microbiology and biotechnology , keratinocyte , protein kinase c , cell adhesion molecule , cadherin , gene isoform , biology , isozyme , cell adhesion , cellular differentiation , kinase , chemistry , in vitro , cell , biochemistry , enzyme , gene
Epidermal expression of adhesion molecules such as desmogleins (Dsg) and cadherins is strongly affected by the differentiation status of keratinocytes. We have previously shown that certain protein kinase C (PKC) isoforms differentially alter the growth and differentiation of human epidermal HaCaT keratinocytes. In this paper, using recombinant overexpression and RNA interference, we define the specific roles of the different PKC isoenzymes in modulation of expression of adhesion molecules in HaCaT keratinocytes. The level of Dsg1, a marker of differentiating keratinocytes, was antagonistically regulated by two Ca‐independent ‘novel’ nPKC isoforms; i.e. it increased by the differentiation‐promoting nPKCδ and decreased by the growth‐promoting nPKCε. The expression of Dsg3 (highly expressed in proliferating epidermal layers) was conversely regulated by these isoenzymes, and was also inhibited by the differentiation inducer Ca‐dependent ‘conventional’ cPKCα. Finally, the expression of P‐cadherin (a marker of proliferating keratinocytes) was regulated by all of the examined PKCs, also in an antagonistic manner (inhibited by cPKCα/nPKCδ and stimulated by cPKCβ/nPKCε). Collectively, the presented results strongly argue for the marked, differential, and in some instances antagonistic roles of individual Ca‐dependent and Ca‐independent PKC isoforms in the regulation of expression of adhesion molecules of desmosomes and adherent junctions in human epidermal keratinocytes.