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Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction
Author(s) -
Saintigny Gaëlle,
Bernard FrançoisXavier,
Juchaux Franck,
Pedretti Nathalie,
Mahé Christian
Publication year - 2008
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2008.00707.x
Subject(s) - contraction (grammar) , microbiology and biotechnology , chemistry , fibroblast , adhesion , human skin , dermal fibroblast , in vitro , biology , biochemistry , endocrinology , genetics , organic chemistry
As revealed by immunohistochemistry and RT‐QPCR, the focal adhesion protein tensin1 is expressed in cultured human dermal fibroblasts and reduced by 60% after transfection with tensin1 siRNA. Tensin1 silenced fibroblast exhibited a strongly reduced capacity to contract collagen gels. Aged fibroblasts, generated with the Hayflick replicative senescence model, exhibit as siRNA silences fibroblasts, a reduced tensin1 expression and an impaired gel contraction capacity. Based on these results, we speculate that in human dermal fibroblasts, tensin1 plays an important role in cell–matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing.