Premium
Absence of amplification of CD4+CD25 high regulatory T cells during in vitro expansion of tumor‐infiltrating lymphocytes in melanoma patients
Author(s) -
Knol Anne Chantal,
Lemaître Fabrice,
Pandolfino Marie Christine,
Volteau Christelle,
Quéreux Gaëlle,
Saiagh Soraya,
Khammari Amir,
Viguier Manuelle,
Dréno Brigette
Publication year - 2008
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2007.00681.x
Subject(s) - foxp3 , melanoma , il 2 receptor , tumor infiltrating lymphocytes , cancer research , in vitro , cd8 , adoptive cell transfer , immunotherapy , immunology , context (archaeology) , biology , t cell , medicine , immune system , paleontology , biochemistry
Abstract: The exact role of CD4+CD25 high regulatory T cells (Treg) in adoptive immunotherapy of melanoma is still to be determined, and an association between an expansion of Treg cells and the expansion of therapeutic tumor‐infiltrating lymphocytes (TIL) remains unelucidated. In this context, the aim of our study was to determine whether functional Treg cells were detectable and amplified among in vitro ‐expanded TIL from 10 metastatic melanoma lymph nodes (LNs). In this study, we investigated the expression of forkhead/winged helix transcription factor 3 (Foxp3) in melanoma‐invaded LNs and determined proportion and functionality of Treg cells among TIL extracted from these 10 metastatic melanoma LNs at different steps of their in vitro expansion. We found that metastatic melanoma LNs expressed very heterogeneous levels of Foxp3 and that CD4+CD25 high Treg cells extracted from these LNs were detectable at each step of the in vitro culture of TIL but decreasing during the culture. In addition, functional assays demonstrated that these CD4+CD25 high T cells were capable of suppressing autologous CD8+ and CD4+CD25‐ T cell proliferation. These cells were indeed Treg cells as they expressed Foxp3. In conclusion, our work suggests that CD4+CD25 high Foxp3 expressing T cells are not expanded during in vitro amplification of TIL obtained from melanoma‐invaded LNs.