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Activation of chloride secretion via proteinase‐activated receptor 2 in a human eccrine sweat gland cell line – NCL‐SG3
Author(s) -
Bovell Douglas L.,
Santic Radmila,
Kofler Barbara,
Hermann Anton,
Wilson Darius,
Corbett Alistair,
Lang Roland
Publication year - 2008
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2007.00659.x
Subject(s) - eccrine sweat gland , secretion , sweat gland , receptor , microbiology and biotechnology , biology , cell culture , epithelium , medicine , sweat , endocrinology , chemistry , biochemistry , genetics
Proteinase‐activated receptor 2 (PAR‐2) has been shown to elicit secretion in a variety of secretory epithelial cells by the transepithelial movement of chloride ions across the apical membrane. However, it is not known whether these receptors are present and/or functional in the secretory epithelial cells of the human eccrine sweat gland. To investigate this possibility mRNA analysis, Ca 2+ microspectrofluorimetry and the short circuit current (Isc) technique were used to quantify electrolyte transport in a cell line (NCL‐SG3) derived from human eccrine sweat gland secretory epithelia. The results provided molecular and functional evidence of the presence of PAR‐2 receptors in the NCL‐SG3 cells and show that these receptors can activate transepithelial Cl − secretion possibly via Ca 2+ ‐activated Cl − channels.