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Detection of down‐regulated acetaldehyde dehydrogenase 1 in atopic dermatitis patients by two‐dimensional electrophoresis
Author(s) -
Park YongDoo,
Lyou YouJeong,
Yang JunMo
Publication year - 2007
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2006.00524.x
Subject(s) - atopic dermatitis , acetaldehyde , dermatology , medicine , chemistry , chromatography , biochemistry , ethanol
We conducted the proteomic studies to detect the dysregulated proteins in the atopic dermatitis (AD) proteome obtained from the patient‐derived primary cultured fibroblasts. Acetaldehyde dehydrogenase 1 (ALDH1) was detected as being significantly down‐regulated at the pH ranges of 6–9 and 4–7. The transcriptional levels of ALDH1, as detected by RT‐PCR and real‐time PCR, further confirmed the down‐regulated phenomena for all the AD‐fibroblasts ( n = 20). The expression levels of ALDH1 in the whole skin tissue samples were further supported by the results of the primary cultured samples. These findings clearly demonstrate that ALDH1 can be a dermal biomarker for AD disease.