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IgG reactivity against non‐conformational NH 2 ‐terminal epitopes of the desmoglein 3 ectodomain relates to clinical activity and phenotype of pemphigus vulgaris
Author(s) -
Müller Ralf,
Svoboda Vera,
Wenzel Elke,
Gebert Susanne,
Hunzelmann Nicolas,
Müller HansHelge,
Hertl Michael
Publication year - 2006
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2006.00451.x
Subject(s) - desmoglein 3 , ectodomain , pemphigus vulgaris , epitope , desmoglein , pemphigus , chemistry , desmoglein 1 , immunology , desmosome , mucocutaneous zone , antibody , autoantibody , medicine , disease , biochemistry , pathology , receptor , cell
Pemphigus vulgaris (PV) is an autoimmune disease caused by immunoglobulin G (IgG) autoantibodies against the desmosomal adhesion molecules, desmoglein (Dsg)3 and Dsg1. The aim of the study was to relate IgG reactivity of 123 PV sera and 40 control sera against NH 2 ‐terminal non‐conformational epitopes of Dsg3 and Dsg1 with disease activity and clinical phenotype by enzyme‐linked immunosorbent assay. The results show that (i) the overall reactivity and the titres of IgG reactive with the Dsg3 ectodomain, Dsg3(1–566), significantly correlated with the disease activity of the PV patients; (ii) IgG reactivity against the NH 2 ‐terminus of Dsg3, Dsg3(1–161), was associated with active PV while there was no direct correlation between the IgG titres and the disease activity; (iii) IgG reactivity against the NH 2 ‐terminus of Dsg3, Dsg3(1–161), was associated with mucosal and mucocutaneous PV; (iv) IgG titres against a small stretch of the NH 2 ‐terminus of Dsg3, Dsg3(25–88), were associated with active PV; and (v) IgG in the PV sera detected non‐conformational epitopes in addition to the previously identified conformation‐dependent epitopes of the Dsg3 and Dsg1 ectodomains.