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The expression of keratinocyte growth factor receptor (FGFR2‐IIIb) correlates with the high proliferative rate of HaCaT keratinocytes
Author(s) -
Nagy Nikoletta,
BataCsörgő Zsuzsanna,
Kopasz Norbert,
Szeg Csilla,
Pivarcsi Andor,
Koreck Andrea,
Dobozy Attila,
Kemény Lajos,
Széll Márta
Publication year - 2006
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.2006.00450.x
Subject(s) - hacat , keratinocyte , fibroblast growth factor receptor 2 , keratinocyte growth factor , cell growth , microbiology and biotechnology , fibroblast , cellular differentiation , receptor , chemistry , biology , growth factor , fibroblast growth factor , cancer research , in vitro , biochemistry , gene
Keratinocyte growth factor receptor (KGFR = FGFR2‐IIIb) is a tyrosine kinase receptor expressed by keratinocytes, which mediates the effects of fibroblast growth factors (FGF). There are contradictory data in the literature regarding the role of FGFR2‐IIIb during the proliferation/differentiation programme of keratinocytes. In this study, we aimed to investigate whether overexpression of FGFR2‐IIIb may have a role in the regulation of keratinocyte proliferation. We analysed the expression of FGFR2‐IIIb in an in vitro HaCaT model system representing different stages of proliferation and differentiation of keratinocytes. Real‐time RT‐PCR and Western blot analyses demonstrated a correlation between FGFR2‐IIIb mRNA and protein expression and the proportion of cells in S/G2/M phase in synchronized HaCaT keratinocytes and thus with proliferation activity ( r = 0.96). After treatment with the antipsoriatic drug, dithranol, FGFR2‐IIIb is downregulated dose dependently both at mRNA and protein levels. Moreover, when the rate of proliferation is decreased by the lack of cell attachment to the culturing surface, FGFR2‐IIIb mRNA ( P = 0.0315) and protein expressions were also reduced ( P = 0.0242), while a differentiation marker, keratin 10, mRNA ( P = 0.0003) and protein levels ( P = 0.001) were increased ( r = −0.92). Based on our results we conclude that FGFR2‐IIIb expression in HaCaT keratinocytes corresponds with the proliferative activation of the cells and is not related to the differentiation programme.