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Skin roughness and wrinkle formation induced by repeated application of squalenemonohydroperoxide to the hairless mouse
Author(s) -
Chiba K.,
Sone T.,
Kawakami K.,
Onoue M.
Publication year - 1999
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1999.tb00305.x
Subject(s) - hairless , squalene , chemistry , erythema , wrinkle , tetradecane , stratum corneum , unsaponifiable , chromatography , biochemistry , dermatology , organic chemistry , materials science , biology , medicine , composite material , genetics
The present study examines the cumulative effects of sub‐erythema application of squalene‐monohydroperoxide (Sq‐OOH), the initial products of UV‐peroxidated squalene, to the skin of hairless mice. Sq‐OOH was isolated by the methanol extraction and preparative HPLC method. Repeated topical application of 10mM Sq‐OOH to hairless mice for 3 weeks induced definite skin roughness and crinkle formation. 3‐D surface parameter analysis revealed changes in all roughness parameters (number of furrows and crests, distance between a furrows and next crest, and irregularity) of the group treated with more than 3 mM Sq‐OOH compared to the control group. Theses skin surface changes were not induced by squalene, squalene‐monohydroxide (Sq‐OH) or organic hydroperxide such as tert ‐butyl hydroperoxide and cumene ‐hydroperoxide at 10mM. Similarly, such changes were not induced by primary irritants, such as sodium lauryl sulfate and n‐tetradecane under the same experimental conditions. Skin conductance decreased, following application of 10 mM Sq‐OOH. Histological observation revealed that application of 10 mM Sq‐OOH induced slight hyperkeratosis, moderate epidermal thickening and slight hyperplasia of sebaceous glands.