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CD24 expression on human keratinocytes
Author(s) -
Redondo Pedro,
GarcíaFoncillas Jesús,
Okroujnov Iouri,
Felipe Iñigo,
Quintanilla Emilio
Publication year - 1998
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1998.tb00320.x
Subject(s) - flow cytometry , cd24 , phorbol , microbiology and biotechnology , cell culture , t cell , cell adhesion molecule , biology , in vitro , immunology , chemistry , protein kinase c , immune system , signal transduction , cd44 , biochemistry , genetics
CD24 or Nectadrin is a cell surface glycoprotein expressed in pre‐B lymphocytes, T lymphocytes, neurons, muscle cells and carcinoma cells. Its function is not completely known, but it has been suggested that it is involved in cell adhesion and signalling. CD24 has recently been identified as the human molecule homologous to the murine heat‐stable antigen (HSA). HSA is expressed by murine keratinocytes and delivers costimulatory signals in T‐cell activation. Long‐term cultures of normal human keratinocytes (HKC) were obtained from skin of human female breast sections and either left untreated or were treated with phorbol‐12‐myristate‐13‐acetate (PMA) at 10–100 ng/ml, calcium 0.5–2 mM or IFN‐γ 100–1000 U/ml, for 24–48 h. Using RT‐PCR and flow cytometry we showed that HKC express low levels of CD24 even under basal conditions, and the treatment with calcium, PMA or IFN‐γ increased levels of CD24 mRNA and protein. To the best of our knowledge, this is the first report to measure CD24 expression and production by cultured HKC in basal conditions and after stimulation. Further studies are needed to determine biological and therapeutical relevance of these findings.