Premium
Serum‐free conditions for the long term growth and differentiation of neoplastic canine keratinocytes *
Author(s) -
Crameri F. M.,
Varvayanis M.,
Cromie B. R.,
Rekers W. L.,
Suter M. M.
Publication year - 1997
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1997.tb00162.x
Subject(s) - keratinocyte , fetal bovine serum , in vitro , epithelium , in vivo , cell culture , biology , chemically defined medium , epidermal growth factor , stratified squamous epithelium , pathology , serum concentration , microbiology and biotechnology , immunology , medicine , endocrinology , biochemistry , genetics
Long term cultures of canine keratinocytes have been established but culture conditions currently used require supplementation with fetal bovine serum (FBS). Unfortunately. FBS contains many non‐defined components which may interfere with in vitro studies. This study describes the development of defined serum‐free culture conditions for neoplastic canine keratinocytes grown submerged and at the air‐liquid interface. Two commercially available serum‐free media established for human epidermal cells failed to support canine keratinocyte growth. In contrast, a defined serum‐free medium developed in our laboratory successfully supported proliferation of neoplastic canine keratinocytes for at least 40 passages. Cells showed a slower growth rate, but reached similar final densities and were morphologically identical to those cultured in FBS. Grown at the air‐liquid interface, the cells reached the same degree of differentiation as in vivo stratified squamous epithelium and cultures grown in FBS. These results demonstrate that canine keratinocytes require different serum‐free growth conditions than human cells. Neoplastic canine keratinocyte cultures, grown under serum‐free culture conditions, provide an ideal in vitro system for comparative studies of keratinocyte biology and pathogenesis of various dermatoses.