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β‐Adrenergic stimulation induces activation of protein kinase C and inositol 1,4,5‐trisphosphate increase in epidermis
Author(s) -
Koizumi Hiroko,
Tanaka Hideo,
Ohkawara Akira
Publication year - 1997
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1997.tb00159.x
Subject(s) - inositol , protein kinase c , phosphatidylinositol , protein kinase a , endocrinology , signal transduction , medicine , cytosol , microbiology and biotechnology , chemistry , biology , receptor , kinase , biochemistry , enzyme
Epidermal keratinocytes express β 2 ‐adrenergic receptors on the cell membrane. The binding of the agonists to the β 2 ‐adrenergic receptors regulates activation of adenylate cyclase. This transmembrane signaling system has been regarded to be one of the important pathways for the functions of keratinocytes. We previously reported that β‐adrenergic stimulation induced a transient increase of intracellular Ca 2+ in normal human epidermal keratinocytes. Thus we investigated the effects of epinephrine on another transmembrane signaling system, the phosphatidylinositol signal transduction pathway in pig epidermis. Treatment of pig pure epidermis with epinephrine resulted in a transient increase in inositol 1,4,5‐trisphosphate with a peak at 30 s. Epinephrine induced translocation of protein kinase C from cytosol to the membrane fraction. The activation of protein kinase C, translocation of protein kinase C from cytosol to the membrane fraction, was confirmed using the β‐adrenergic agonist isoproterenol. Moreover, the effect of epinephrine on the activation of protein kinase C was inhibited by preincubation with propranolol, a β‐adrenergic antagonist. The increase in inositol 1,4,5‐trisphosphate and translocation of protein kinase C by epinephrine are consistent with the view that β‐adrenergic stimulation induces turnover of inositol phospho‐lipid in pig epidermis.