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Establishment and characterization of a novel human cell line exhibiting both immunophenotypic markers of monocyte/macrophage and natural killer cell lineages from peripheral blood of a patient with atopic dermatitis
Author(s) -
Hamamoto Y.,
Nagai K.,
Muto M.,
Nakamura K.,
Yasui H.,
Asagami C.
Publication year - 1997
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1997.tb00145.x
Subject(s) - peripheral blood mononuclear cell , ficoll , population , immunology , biology , natural killer cell , alkaline phosphatase , atopic dermatitis , eosinophil peroxidase , immunophenotyping , cell culture , lymphocyte , cd3 , microbiology and biotechnology , in vitro , flow cytometry , biochemistry , medicine , immune system , cytotoxicity , myeloperoxidase , enzyme , inflammation , cd8 , genetics , environmental health
Large amounts of homogeneous cells are not always available for in vitro studies of inflammatory skin disorders. Here we demonstrate that a novel cell line, termed YAA, has been established from peripheral blood mononuclear cells, which were separated by the Ficoll method, of a patient with atopic dermatitis. YAA cells were grown in suspension culture. The cytochemical staining showed a positive reaction for α‐naphthyl butyrate esterase, which was completely inhibited by sodium fluoride, but a negative result for periodic acid‐Schiff, peroxidase and alkaline phosphatase. A large population of YAA cells exhibited phenotype of CD33 and CD56 but neither of CD2 nor CD3. The phorbol ester‐stimulated YAA cells produced a considerable amount of tumor neerosis factor‐α. These findings suggest that YAA might be a monocytoid line with an additional phcnolype specific for natural killer cells.