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Attachment, spreading and migration of melanoma cells on vitronectin
Author(s) -
Leeuwen Robert L.,
Yoshinaga Iara G.,
Akasaka Toshihide,
Dekker Sybren K.,
Vermeer Bert Jan,
Byers H. Randolph
Publication year - 1996
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1996.tb00134.x
Subject(s) - vitronectin , integrin , cell migration , alpha v beta 3 , cell culture , flow cytometry , microbiology and biotechnology , cell , receptor , biology , cell adhesion , chemistry , cancer research , biochemistry , genetics
Recent in situ studies suggest the α v β 3 integrin is a tumour progression marker in melanoma. We analyzed 5 human melanoma cell lines for their expression of the vitronectin binding α v β 3 and α v β 5 , integrins using flow cytometry. The role of these receptors in cell attachment, spreading and migration was investigated using attachment assays, video time lapse spreading and migration assays and with function blocking monoclonal antibodies. Cell lines derived from later stages of tumor progression exhibited high levels of α v β 3 expression, whereas no similar correlation with α v β 5 expression was identified. Cell attachment, spreading and migration response on vitronectin correlated well with the expression level of the α v β 3 but not the α v β 5 vitronectin receptor. Blocking of the α v β 3 integrin resulted in a significant decrease in cell attachment, spreading and motility whereas the function blocking antibody against the α v β 5 integrin only inhibited cell attachment in cell lines with the highest level of expression of this integrin. Taken together, our study indicates that the level of expression of the α v β 3 and α v β 5 integrins is heterogeneous in melanoma cell lines and that the α v β 5 integrin, if present, may function only during the initial cell attachment whereas the α v β 3 plays an important rôle in cell spreading and cell migration as well.