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UVA‐induced oxidative damage in fibroblasts cultured in a 3‐dimensional collagen matrix
Author(s) -
Coulomb B.,
Lebreton C.,
Mathieu N.,
Morlière P.
Publication year - 1996
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1996.tb00111.x
Subject(s) - lipid peroxidation , chemistry , incubation , fibroblast , oxidative damage , vitamin e , matrix (chemical analysis) , antioxidant , dermal fibroblast , biochemistry , vitamin c , thiobarbituric acid , biophysics , microbiology and biotechnology , in vitro , biology , chromatography
The exposure of dermal equivalent, i.e., fibroblasts cultured in a 3‐dimensional collagen matrix to realistic doses of UVA (≤ 200 kJ/m 2 ), results in a lipid peroxidation process as evidenced by the release of thiobarbituric acid‐reactive substances in the supernatant. This peroxidalive process is shown to be associated with the presence of libroblasts and is inhibited by pre‐incubation with vitamin E. the well‐known chain‐breaking antioxidant. Moreover, the UVA irradiation triggers cyclotoxic effects which can also be reversed by preincubation with vitamin E. While the peroxidation extent is similar for fibroblasts cultured in monolayers or in dermal equivalent, the cytotoxic response to UVA is more pronounced in dermal equivalent.