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Effects of UVB irradiation on keratinocyte growth factor (KGF) and receptor (KGFR) expression in cultured human keratinocytes
Author(s) -
Zhou Youwen,
Lee HyungSuk T.,
Kooshesh Fatemeh,
Fujisawa Hiroshi,
Sauder Daniel N.,
Kondo Seiji
Publication year - 1996
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1996.tb00108.x
Subject(s) - keratinocyte growth factor , keratinocyte , downregulation and upregulation , messenger rna , biology , reverse transcription polymerase chain reaction , cancer research , receptor , endocrinology , growth factor , cell culture , gene , biochemistry , genetics
Keratinocyte growth factor (KGF) and its receptor (KGFR) are thought to play important roles in normal keratinocyte growth and differentiation. Since UVB radiation is known to influence keratinocyte growth, we sought to determine whether UVB would alter the expression of KGF and KGFR. Using a reverse‐transcription coupled polymerase chain reaction (RT‐PCR). the present study examined the expression of KGF and KGFR mRNA in cultured normal human keratinocytes exposed to UVB irradiation. Total cellular RNA was extracted from cultured keratinocytes at various time points after irradiation, reverse transcribed and used for PCR amplification using primers specific for KGF and KGFR. Constitutive expression of KGFR mRNA, but not KGF mRNA, was detected in normal cultured human keratinocytes. After UVB irradiation at 300 J/m 2 , the KGF mRNA remained undelectable while the KGFR mRNA level was significantly decreased. The downregulation of KGFR mRNA expression was also confirmed by Northern blot analysis. Immunohistochemical studies demonstrated a decreased positive signal of KGFR in human keratinocytes after UVB irradiation. Our results suggest a possible role for the KGF‐KGFR signalling pathway in the skin after exposure to UVB, and that UVB‐induced growth inhibition of keratinocytes in hyperproliferative skin disorders may be related to downregulation of KGFR.