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Retinoic acid induces expression of PA‐FABP (psoriasis‐associated fatty acid‐binding protein) gene in human skin in vivo but not in cultured skin cells
Author(s) -
Larsen Frederik Grønhøj,
Voorhees John J.,
Åström Anders
Publication year - 1994
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1994.tb00279.x
Subject(s) - retinoic acid , in vivo , psoriasis , fatty acid binding protein , human skin , gene expression , chemistry , in vitro , gene , biochemistry , microbiology and biotechnology , biology , immunology , genetics
PA‐FABP (psoriasis‐associated fatty acid binding protein) is a new member of a group of low‐molecular‐weight proteins that are highly up regulated in psoriatic skin and that share similarity to fatty acid‐binding proteins. In this study we demonstrate that PA‐FABP transcripts are expressed in human skin in vivo and that topical application of 0.05% retinoic acid (RA) cream results in a rapid induction of PA‐FABP transcripts following treatment for 16 hours and persists at increasing levels after 48 and 96 h of RA treatment. The PA‐FABP mRNA response to RA was reduced by approximately 50% when patients concurrently were treated with RA and 0.025% clobelasol propionate (CLO) for 48 and 96 h, whereas treatment with CLO alone resulted in PA‐FABP transcript levels not significantly different from vehicle‐treated skin. When comparing the effects of a well‐known irritant, sodium lauryl sulfate (SLS), to those of RA and its vehicle, 0.05% RA cream but not 2% SLS in RA vehicle caused PA‐FABP mRNA induction after 16 h. SLS treatment of human skin for 96 h caused a slight increase in PA‐FABP transcripts, but markedly less than that observed in response to RA treatment. Incubation of cultured human keratinocytes or skin fibroblasts with RA for up to 48 h did not significantly induce PA‐FABP transcripts. Expression of PA‐FABP message in keratinocytes was observed to be induced by calcium and fetal calf serum (FCS), while tetra‐decanoyl phorbol acetate (TPA) caused little or no induction. Taken together, the marked inducibility of the PA‐FABP gene is compatible with the possibility that this gene might be important in RA‐mediated regulation of human skin growth and differentiation.