Premium
Immunocytochemical detection of the carbohydrate antigen, Sialyl Lewis x , in normal human skin and during irritant contact dermatitis
Author(s) -
Mikulowska Anna,
Ohlsson Lennart,
Boketoft Åke,
Andersson Anders,
Falck Bengt
Publication year - 1994
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1994.tb00277.x
Subject(s) - epidermis (zoology) , dermis , immunocytochemistry , contact dermatitis , monoclonal antibody , chemistry , sialyl lewis x , antigen , langerhans cell , microbiology and biotechnology , pathology , antibody , immunology , medicine , biology , allergy , cell adhesion molecule , anatomy , selectin
Sialys Lewis x (SLex) is a ligand for the E‐selectin and the interaction of E‐selectin on the endothelium and SLe x on T cells may be important for T‐cell migration into the skin. We investigated the expression of SLe x on Langerhans cells (LC) in normal skin and on LC repopulating epidermis deprived of LC due to a preceding irritant contact dermatitis. SLe x was visualized by fluorescence and light microscopic immunocytochemistry using the monoclonal antibody. CSLEX‐1. The results showed that about 40% of LC in normal epidermis express SLe x . In the repopulation phase, most of the epidermal cells were CDla + /SLe x+ . We suggest that SLe x is present on epidermal LC that have recently immigrated from the dermis.