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Immunohistochemical study of purulent wounds treated with King crab collagenase
Author(s) -
Sakharov I. Yu.,
Shekhonin B. V.,
Glyanzev S. P.,
Litvin F. E.
Publication year - 1994
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1994.tb00046.x
Subject(s) - collagenase , granulation tissue , fibrin , fibronectin , polyclonal antibodies , immunohistochemistry , wound healing , protease , pathology , chemistry , biology , microbiology and biotechnology , antibody , medicine , extracellular matrix , enzyme , immunology , biochemistry
Immunohistochemical study of tissues from purulent wounds in rats after treatment with the collagenase isolated from the King crab Paralithodes camtschitica was undertaken. The enzymotherapy resulted in a rapid and efficient removal of necrotic debris. It was accompanied by fibrin elimination from the wound bed and subsequent formation of new capillaries. Cellular fibronectin with ED‐A sequence was identified in the newly formed granulation tissue, which points to its active synthesis in situ . Polyclonal antibodies against two isozymes of the crab collagenolytic protease were obtained. By their use it was shown that, after application of the collagenase, both isozymes accumulated in fibrin deposits at the wound bed but did not penetrate adherent granulation tissue.