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Production of multiple cytokines by cultured human melanomas *
Author(s) -
Bennicelli Jeannette L.,
Guerry DuPont
Publication year - 1993
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1993.tb00031.x
Subject(s) - cytokine , in vivo , melanoma , cell culture , secretion , tumor necrosis factor alpha , immunology , interleukin 6 , cancer research , in vitro , transforming growth factor , biology , medicine , biochemistry , microbiology and biotechnology , genetics
We screened a panel of 8 primary and 21 metastatic melanoma cell lines for constitutive secretion of cytokines. Melanomas expressed bioactivity for TGF‐β (8/25 lines) and IFN (7/12), but not IL‐2. Immu‐noassays detected IL‐1α (4/25), IL‐1β (12/25), 1L‐6 (13/29), IL‐8 (29/ 29), TGF‐β 2 , (5/12) and GM‐CSF (11 /29). but not IL‐3, IL‐4, TNF‐α, or IFN‐γ. There was no preferential association of cytokine production with cells cultured from primary versus metastatic disease, and only IL‐8 was produced by all lines tested. These data demonstrate that cultured melanomas produce a variety of cytokines which are potentially capable of influencing tumor growth in vivo.