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UVA does not photoreactivate pyrimidine dimers in cultured human fibroblasts
Author(s) -
Tanew Adrian,
Ortel Bernhard,
Hönigsmann Herbert
Publication year - 1993
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1993.tb00026.x
Subject(s) - pyrimidine dimer , pyrimidine , human skin , irradiation , microbiology and biotechnology , chemistry , in vitro , endonuclease , cell culture , biology , biophysics , dna , biochemistry , dna damage , genetics , physics , nuclear physics
Pyrimidine dimers were induced in duplicates of cultured human skin fibroblasts by irradiation with various doses of UVB radiation. Subsequently, one set of cells was further exposed to either 5 or 10 J/cm 2 of UVA radiation to assess the photoreactivating activity of this spectral range in a human cell system. Following irradiation, pyrimidine dimers were quantified in all cells by determining the number of endonuclease‐sensitive sites (ESS). No difference in the yield of ESS was observed between cells which had been irradiated with UVB only as compared to cells which subsequently had been exposed to 5 or 10 J/cm 2 UVA. In contrast, subsequent exposure of UVB‐irradiated cells of Monodelphis domestica to 10 J/cm 2 UVA resulted in an almost 50% reduction of UVB‐induced pyrimidine dimers. These data indicate that UVA does not induce photoenzymatic repair in human fibroblasts.