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Chemical carcinogens increase IL‐1α and IL‐6 gene transcripts in human keratinocytes
Author(s) -
Khan Islam U.,
Mukhtar Hasan,
Haqqi Tariq M.
Publication year - 1993
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1993.tb00013.x
Subject(s) - carcinogen , gene , biology , keratinocyte , gene expression , genetics , immunology , cancer research , microbiology and biotechnology , chemistry , cell culture
Using reverse transcriptase‐linked polymerase chain reaction, the effect of polycyclic aromatic hydrocarbons (PAHs) on IL‐lα, IL‐lβ and IL‐6 gene expression in cultured human keratinocytes was studied. Exposure to β‐naphthoflavone and benz(a)anthracene resulted in a higher copy number of IL‐lα and IL‐6 mRNA while lower level of IL‐1β mRNA was detected in these cells. These data suggest that, like ultraviolet B (UVB) radiation, ubiquitous environmental carcinogenic PAHs are potent inducers of lL‐lα and IL‐6 cytokines and, unlike UVB, they downregulate IL‐1β in human keratinocytes.