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The ELAM‐1 ligand sialosyl‐Le x is present on Langerhans cells isolated from stratified epithelium
Author(s) -
Nylander Karin,
Larsen Jorgen K.,
Larsen Jacob,
Clausen Henrik,
Bonding Nina,
Dabelsteen Erik
Publication year - 1992
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/j.1600-0625.1992.tb00082.x
Subject(s) - immunohistochemistry , immunostaining , antigen , epithelium , flow cytometry , microbiology and biotechnology , chemistry , dendritic cell , biology , langerhans cell , pathology , immunology , medicine
In this study we show the expression of the newly identified carbohydrate ligand. sialosyl‐Le x on Langerhans cells. The receptor Tor sialosyl‐Le x is the endothelial leukocyte adhesion molecule‐1 (ELAM‐1) present on activated endothelial cells. Using How cytometry. Langerhans cells were selected due to positivity for an antibody against CD la and low orthogonal light scatter. The CD la antigen stained by the OK T6 antibody is considered a maturational marker of Langerhans cells in agreement with the specific labeling of dendritic cells in the epithelium only. Double immunostaining (OKT6/anti‐sialosyl‐Le x ) using flow cytometry and immunohistochemistry demonstrated that almost all OKT6‐positive cells in normal stratified epithelium expressed sialosyl‐Le x . Conversely, by immunohistochemistry of oral epithelium with acute inflammation, additional dendritic cells negative for OKT6 were found to express sialosyl‐Le x In addition, sialosyl‐Le x ‐positive but not OKT6‐positive dendritic cells were found in the submucosa. These findings indicate that the carbohydrate antigen sialosyl‐Le x is expressed earlier than the CD la antigen in the maturation of the Langerhans cell lineage. Future studies should aim at investigating the importance of adhesion between sialosyl Le x and ELAM‐1 in epithelial recruitment of Langerhans cells.