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Predictors of histology, tissue eosinophilia and mast cell infiltration in Hodgkin’s Lymphoma – a population‐based study
Author(s) -
Glimelius Ingrid,
Rubin Jenny,
Rostgaard Klaus,
Amini RoseMarie,
Simonsson Martin,
Sorensen Karina M.,
Smedby Karin E.,
Venge Per,
Hjalgrim Henrik,
Molin Daniel,
Enblad Gunilla
Publication year - 2011
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.2011.01652.x
Subject(s) - nodular sclerosis , eosinophilia , eosinophil , medicine , lymphoma , univariate analysis , population , asthma , histology , odds ratio , immunology , pathology , gastroenterology , multivariate analysis , hodgkin lymphoma , environmental health
Abstract Objective: Classical Hodgkin’s lymphoma (HL) lesions comprise few tumour cells, surrounded by numerous inflammatory cells. Like in other malignancies, the microenvironment is presumed to be clinically important in HL; however, microenvironment predictors remain poorly characterised. The aim of this study was to investigate how selected patient characteristics and genetic factors affect HL phenotype, in particular tissue eosinophilia, mast cell counts and HL histological subtype. Methods: In a population‐based study, patients with HL were interviewed about potential HL risk factors. Available tumours, n = 448, were classified histologically; the number of eosinophils and mast cells were estimated, and eosinophil cationic protein (ECP) and eosinophil protein‐x (EPX) gene polymorphisms were determined. Associations were assessed in regression models. Results: Self‐reported history of asthma was predictive of having tumour eosinophilia [≥200 eosinophils/10 high power fields, univariate odds ratio (OR) = 2.22, 95% CI 1.06–4.64, P = 0.03]. High numbers of eosinophils were predominantly seen in patients carrying the genotype ECP434GG [multivariate relative levels (RLs) = 1.84, 95% CI 1.02–3.30, P = 0.04]. Lower number of eosinophils was seen in Epstein–Barr virus (EBV)‐positive tumours (univariate RL = 0.52, 95% CI 0.3–0.9, P = 0.02) and in older patients (univariate RL = 0.85, 95% CI 0.73–0.99, P = 0.03). Well‐known factors such as young age, female sex and EBV‐negative status predicted nodular sclerosis histology. Conclusion: The number of eosinophils in HL tumours is influenced by patient traits such as asthma, ECP genotype and EBV status. EBV status was predictive of histology.