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Removal of autologous activated CD4‐positive T lymphocytes also results in increased colony‐forming units in patients with low and intermediate‐1 risk myelodysplastic syndromes
Author(s) -
Zheng Zhang,
Feng Xu,
Xiao Li,
Qianqiao Zhang,
Qi He,
Lingyun Wu
Publication year - 2011
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.2010.01535.x
Subject(s) - haematopoiesis , in vitro , bone marrow , immunology , biology , andrology , progenitor cell , microbiology and biotechnology , myelodysplastic syndromes , trisomy 8 , cd34 , stem cell , medicine , chromosome , karyotype , biochemistry , gene
Autologous activated CD4 + T lymphocytes (CD4 + /CCR5 + double‐positive cells) that derived from BMNCs of patients with low and intermediate‐1 risk myelodysplastic syndrome were depleted or added to in vitro cultures. The BMNCs depleted of CD4 + /CCR5 + T cells exhibited significantly increased numbers of colony‐forming units (CFUs). Conversely, the bone marrow mononuclear cells cultures with a fourfold augmentation of CD4 + /CCR5 + T lymphocytes exhibited no colonies in cultures in vitro . The apoptotic index (AI) of colony cells was decreased compared with that of preculture counterparts. After depletion of CD4 + /CCR5 + in vitro cultures, the clonal cells increased in patients with chromosome 5q‐ or 20q‐ abnormalities but remained unchanged in patients with trisomy 8. In addition, after removal of CD4 + /CCR5 + T cells, the number of CFUs was increased in those patients with a higher number of BM Th1 (CD4 + / IFN‐γ + ) cells, hypocellularity, or bearing the DR15 allele. We concluded that the selective removal of autologous activated CD4 + T cells can increase the generation of CFUs. However, whether the increased CFUs consisted of cells derived from residual normal hemopoiesis or clonal hemopoiesis remains unknown.