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U‐2973, a novel B‐cell line established from a patient with a mature B‐cell leukemia displaying concurrent t(14;18) and MYC translocation to a non‐ IG gene partner
Author(s) -
Boström Hans,
Leuchowius KarlJohan,
Hallböök Helene,
Nordgren Ann,
Thörn Ingrid,
Thorselius Mia,
Rosenquist Richard,
Söderberg Ola,
Sundström Christer
Publication year - 2008
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.2008.01098.x
Subject(s) - chromosomal translocation , karyotype , biology , fluorescence in situ hybridization , b cell , microbiology and biotechnology , leukemia , bone marrow , cancer research , gene rearrangement , lymphoma , cytogenetics , chromosome , gene , genetics , antibody , immunology
B‐cell lymphomas/leukemias with simultaneous t(14;18)(q32;q21) and MYC rearrangements have recently been shown to constitute a separate diagnostic entity, presenting with a rapid clinical course and a very poor prognosis. We describe the establishment of an Epstein–Barr virus negative cell line, designated U‐2973, from a male patient with a de novo aggressive B‐cell lymphoma/leukemia and very high peripheral blast cell count. Flow cytometry of bone marrow cells and U‐2973 displayed a mature B‐cell phenotype, and immunostaining showed expression of MYC and BCL2. IG gene rearrangement data were consistent with a lymphoid neoplasm of germinal centre derivation. Cytogenetic studies using conventional G‐banding, fluorescent in situ hybridization, spectral karyotyping and single nucleotide polymorphism array demonstrated a complex karyotype with both a t(14;18) and double translocations between MYC and a non‐ IG gene partner located at chromosome 12p12.1.