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Evaluation of the prognostic relevance of l ‐selectin and ICAM1 expression in myelodysplastic syndromes
Author(s) -
Buccisano Francesco,
Maurillo Luca,
Tamburini Anna,
Del Poeta Giovanni,
Del Principe Maria Ilaria,
Ammatuna Emanuele,
Consalvo Maria Irno,
Campagna Selenia,
Ottaviani Licia,
Sarlo Chiara,
Renzi Daniela,
Faccia Sabrina,
Fraboni Daniela,
Coco Francesco Lo,
Amadori Sergio,
Venditti Adriano
Publication year - 2008
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.2007.00986.x
Subject(s) - cd34 , myelodysplastic syndromes , bone marrow , myeloid , flow cytometry , medicine , progenitor cell , cancer research , l selectin , infiltration (hvac) , stem cell , biology , cell adhesion molecule , immunology , genetics , physics , thermodynamics
Objectives: An aberrant pattern of expression of l ‐selectin and intercellular adhesion molecule 1 (ICAM1) may characterise CD34 + blast cells in myelodysplastic syndromes (MDS) and secondary acute myeloid leukaemia (sAML). Methods: In a three‐colour flow cytometric assay, we evaluated the expression of l ‐selectin and ICAM1 on CD34 + blast cells from the bone marrow (BM) of 66 MDS patients; for the purpose of comparison CD34 + blast cells of 18 sAML and CD34 + stem cells of 17 normal donors were also analysed. Results: The ratio of l ‐selectin/ICAM1 expression was identified as a parameter correlated with the percentage of BM blast infiltration and the time to leukaemic progression among MDS patients. In fact, the values of l ‐selectin/ICAM1 ratio were inversely correlated with the BM blast infiltration ( r = –0.34, P = 0.004). Furthermore, MDS patients with a baseline ratio <1 had a higher leukaemic progression rate (41% vs. 19%, P = 0.008); the actuarial risk of disease progression for this subgroup of MDS patients was also higher (64% vs. 11% at 2 yr, P = 0.002). Furthermore, in two patients a decrease of the ratio was observed when overt leukaemic transformation occurred; conversely, restoration of a normal ratio was observed in two patients after a chemotherapy‐induced remission. Conclusion: (i) l ‐selectin is defective in the stem cell compartment of MDS and sAML, whereas ICAM1 is overexpressed; (ii) the ratio of their expression has a prognostic role; and (iii) a ratio <1 significantly predicts progression to overt leukaemia in MDS patients.