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Interleukin‐5 does not influence differential transcription of transmembrane and soluble isoforms of IL‐5R α in vivo
Author(s) -
Byström Jonas,
Dyer Kimberly D.,
Ravin Suk See TingDe,
Naumann Nora,
Stephany David A.,
Foster Paul S.,
Wynn Thomas A.,
Rosenberg Helene F.
Publication year - 2006
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.2006.00699.x
Subject(s) - biology , microbiology and biotechnology , rna splicing , alternative splicing , transmembrane protein , transcription (linguistics) , gene , gene isoform , reverse transcriptase , in vivo , gene expression , transmembrane domain , receptor , genetics , rna , linguistics , philosophy
Interleukin‐5 (IL‐5) promotes signal transduction and expansion of eosinophil colonies in bone marrow via interactions with its heterodimeric receptor (IL‐5R). Two variants encoding soluble forms of the alpha subunit (sIL‐5R α ) have been described, although the signals promoting and/or limiting differential transcription remain to be clarified. Objectives : Our intent was to explore the role of IL‐5 in regulating differential transcription of these splice variants in vivo . Methods : We have designed a quantitative reverse transcriptase‐polymerase chain reaction assay to detect transcripts encoding the transmembrane, soluble 1 and 2 forms of IL‐5R α in two strains of wild‐type (BALB/c and C57BL/6) and corresponding IL‐5 gene‐deleted mice. Wild‐type mice respond to S. mansoni infection with a gradual increase in serum IL‐5 and eosinophilia, which is not observed in IL‐5 gene‐deleted mice. Results and conclusions : We find that IL‐5 is not necessary for differential splicing to occur in vivo , as all three forms of the IL‐5R α are detected in both strains of IL‐5 gene‐deleted mice, with ratios of transcript expression (transmembrane : soluble 1 : soluble 2) that were indistinguishable from their wild‐type counterparts. Differential splicing does vary markedly between strains, potentially because of local effects of strain‐specific polymorphisms.