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Human marrow mesenchymal stem cell culture: serum‐free medium allows better expansion than classical α‐MEM medium
Author(s) -
Meuleman Nathalie,
Tondreau Tatiana,
Delforge Alain,
Dejeneffe Marielle,
Massy Martine,
Libertalis Mark,
Bron Dominique,
Lagneaux Laurence
Publication year - 2006
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.2005.00611.x
Subject(s) - mesenchymal stem cell , progenitor cell , haematopoiesis , bone marrow , peripheral blood mononuclear cell , stem cell , microbiology and biotechnology , chemistry , immunology , biology , andrology , in vitro , medicine , biochemistry
The expansion of mesenchymal stem cells (MSCs) strongly depends on the culture conditions and requires medium supplemented with 10–20% fetal calf serum (FCS) to generate relevant numbers of cells. However, the presence of FCS is a major obstacle for their clinical use. Therefore, we have evaluated the capacity of expansion of MSC in a commercial serum‐free medium (UC) supplemented with a serum substitute (ULTROSER®) in comparison with a classical medium α ‐MEM containing 15% FBS. Bone marrow‐mononuclear cells collected from 12 volunteer healthy donors were expanded in two different culture media. MSCs isolated in the both media were morphologically similar and expressed identical phenotypic markers. After the primoculture (P0) and one passage, we obtained significantly more MSC and CFU‐F progenitors in UC medium than in α MEM. Their multipotentiality was preserved during culture, as well as their capacity to support haematopoiesis. In conclusion, our observations strongly suggest that UC is an optimal medium for ex vivo expansion of MSC: it allows a better cell expansion, preserves cell multipotentiality, reduces the culture period and contains low concentration of serum substitute. This medium seems suitable for clinical scale expansion of MSC.