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Circulating endothelial cells in patients with acute myeloid leukemia
Author(s) -
Wierzbowska Agnieszka,
Robak Tadeusz,
Krawczyńska Anna,
WrzesieńKuś Agata,
Pluta Agnieszka,
Cebula Barbara,
Smolewski Piotr
Publication year - 2005
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.2005.00549.x
Subject(s) - medicine , myeloid leukemia , gastroenterology , white blood cell , flow cytometry , myeloid , leukemia , angiogenesis , progenitor cell , chemotherapy , immunology , stem cell , biology , genetics
Objectives : The circulating endothelial cells (CEC) are proposed to be a non‐invasive marker of angiogenesis. The level of CEC in peripheral blood (PB) of acute myeloid leukemia (AML) patients has not been investigated prior to this study. We evaluated the count of resting (rCEC), activated (aCEC) and endothelial progenitor cells (CEPC) in the PB of AML and healthy subjects. In addition we correlated the levels of CEC with disease status, known prognostic factors and response to treatment. Methods : CEC were quantified by utilizing four‐color flow cytometry procedures in 48 AML patients at the time of diagnosis and 29 healthy controls. Additionally, measurements were again taken after the first course of induction treatment in 12 of the patients. Results : The numbers of aCEC, rCEC and CEPC were significantly higher in the AML patients than in the controls ( P < 0.0001, P < 0.0001 and P < 0.001, respectively). The CEC count was significantly higher in the AML patients with white blood cell count (WBC) >15 G/L, elevated lactic dehydrogenase (LDH) levels and a higher (over median) absolute blasts count (ABC) in PB than in the group with WBC <15 G/L ( P < 0.03), a normal LDH level ( P < 0.03) and a lower (