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Platelet and granulocyte alloimmunisation in multitransfused Tunisian patients
Author(s) -
Skouri Hadef,
Bettaieb Ali,
Fromont Patricia,
Elomri Halima,
Ennabli Souad,
Bierling Philippe
Publication year - 2005
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.2005.00494.x
Subject(s) - thrombasthenia , platelet , immunofluorescence , granulocyte , medicine , agglutination (biology) , antibody , immunology , antigen , glanzmann's thrombasthenia , platelet transfusion , monoclonal antibody , platelet membrane glycoprotein , isoantibodies , myelodysplastic syndromes , platelet aggregation , bone marrow
We determined the frequency of post‐transfusion alloimmunisation against platelet and granulocyte antigens in 51 Tunisian polytransfused patients with haematological diseases. Serum samples were analysed by a standard and an antiglobulin‐augmented lymphocytotoxicity technique, a granulocyte agglutination test, a granulocyte immunofluorescence test, a platelet immunofluorescence test and the monoclonal antibody‐specific immobilisation of platelet antigens assay. No granulocyte‐specific antibodies were detected. HLA antibodies were found in 58.8% of patients. Platelet‐specific antibodies were detected in four patients and were directed against human platelet antigen (HPA)‐5b, HPA‐1b and HPA‐3a. The three patients with Glanzmann's thrombasthenia developed anti‐GPIIb/IIIa antibodies. This study provides immunogenetic information that could improve the management of transfusion therapy in Tunisia.