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Specific antibodies to mouse Sca‐1‐ (Ly‐6A/E) or Thy‐1‐positive haematopoietic progenitor cells induce formation of nitric oxide which inhibits subsequent colony formation
Author(s) -
Bøyum Arne,
Fjerdingstad Hege B.,
Tennfjord ViviAnn,
Benestad Haakon B.,
Løvhaug Dagfinn
Publication year - 2004
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.2004.00322.x
Subject(s) - haematopoiesis , progenitor cell , stem cell , monoclonal antibody , cd90 , microbiology and biotechnology , biology , population , bone marrow , antibody , lymphopoiesis , nitric oxide , immunology , chemistry , cd34 , endocrinology , medicine , environmental health
Mouse bone marrow cells were exposed to specific monoclonal antibodies, so that lineage positive (Lin + ) cells could be removed with magnetic beads. The Lin − cells were cultured with Sca‐1 or CD90 (Thy‐1) monoclonal antibodies (MoAbs) in semi‐solid medium for 7 d. We found that Sca‐1 MoAb suppressed colony formation (20–30%), and the effect was largely abolished by N‐nitro‐ l ‐arginine methyl ester ( l ‐NAME), an inhibitor of nitric oxide (NO) synthase. Similar results were obtained with antibodies to CD90. The findings suggest that the unknown physiological ligands to Sca‐1 and Thy‐1 markers on haematopoietic progenitor cells can inhibit colony formation, with NO as a pivotal mediator. Primitive progenitors may be a primary target of this Sca‐1 ligand, as the Sca‐1 + cell population contains the major part of the multipotent haematopoietic stem cells.