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Involvement of SHP‐1, a phosphotyrosine phosphatase, during myeloid cell differentiation in acute promyelocytic leukemia cell lines
Author(s) -
Uesugi Yumiko,
Fuse Ichiro,
Toba Ken,
Kishi Kenji,
Furukawa Tatsuo,
Koike Tadashi,
Aizawa Yoshifusa
Publication year - 1999
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1999.tb01753.x
Subject(s) - myeloid leukemia , retinoic acid , myeloid , hl60 , cellular differentiation , cell culture , cell growth , downregulation and upregulation , acute promyelocytic leukemia , cancer research , tretinoin , biology , leukemia , microbiology and biotechnology , chemistry , immunology , biochemistry , gene , genetics
We investigated the modulation of the expression and phosphatase activity of SHP‐1 during granulocytic differentiation of human myeloid leukemia cell line, HL60 and t(15;17) positive APL cell line, HT93, in response to all‐trans retinoic acid (ATRA) or dimethylsulfoxide (DMSO). ATRA induced differentiation in both cell lines which was associated with marked growth inhibition and S‐phase reduction. On the other hand, DMSO induced it only in HL60 without obvious growth inhibition and S‐phase reduction. The expression and phosphatase activity of SHP‐1 were upregulated only when the 2 cell lines were differentiated to granulocytes. Furthermore, the changes were not dependent on the inducers or the growth inhibition. These findings suggest that SHP‐1 is involved in common myeloid differentiation, and that upregulation of SHP‐1 is not always related to myeloid cell growth.