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A comparative study of CD34 + cells, CD34 + subsets, colony forming cells and cobblestone area forming cells in cord blood and bone marrow allografts
Author(s) -
TheilgaardMönch Kim,
RaaschouJensen Klas,
Heilmann Carsten,
Andersen Helle,
Bock Johannes,
Russel Charlotte A.,
Vindeløv Lars,
Jacobsen Niels,
Dickmeiss Ebbe
Publication year - 1999
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1999.tb01741.x
Subject(s) - cd34 , cord blood , transplantation , population , bone marrow , progenitor cell , haematopoiesis , flow cytometry , cd38 , andrology , biology , microbiology and biotechnology , immunology , stem cell , medicine , environmental health
Cord blood (CB) has become an alternative source of hematopoietic progenitor cells (HPCs) for allogeneic transplantation. We have developed a new efficient protocol for CB collection. Using this method an average of 17.7 times 10 8 [range (6.8–29.6) × 10 8 , n = 13] total nucleated cells (TNCs) were harvested. Based on recent Eurocord data, which have shown safe engraftment using a threshold dose of 0.37 times 10 8 CB TNCs/kg body weight (BW), we calculated that six out of thirteen CB grafts collected by this method were sufficient to engraft adults. The CB derived CD34 + population contained two‐fold higher numbers of committed HPCs (CFU‐GM, BFU‐E) and six‐fold higher numbers of pluripotent HPCs [CD34 + /CD38 − cells, wk 5 and wk 8 cobblestone area forming cells (CAFCs)] than the CD34 + population of BM. Extrapolation revealed that BM grafts providing the threshold dose for allogeneic transplantation of 2 times 10 8 TNCs/kg BW contained nearly 3 times more pluripotent HPCs than CB grafts providing the Eurocord threshold dose. The assessment of CD34 + /CD38 − cell numbers in CB grafts was highly reproducible and correlated well with the in vitro performance of pluripotent HPCs, i.e. numbers of CAFCs. We conclude that CB grafts providing high numbers of TNCs have the potential to engraft adults and that the enumeration of pluripotent HPCs by flow cytometry may be a useful tool to define the ultimate threshold dose for CB transplantation.