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Flt‐3 ligand does not induce the growth of peripheral blood granulocyte‐macrophage colony‐forming cells in myeloproliferative disorders
Author(s) -
Siitonen T.,
Savolainen E.R.,
Koistinen P.
Publication year - 1999
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1999.tb01729.x
Subject(s) - polycythaemia , cfu gm , granulocyte macrophage colony stimulating factor , progenitor cell , immunology , granulocyte , granulocyte colony stimulating factor , colony stimulating factor , growth factor , myeloproliferative disorders , stem cell factor , interleukin 3 , haematopoiesis , cytokine , biology , cancer research , medicine , stem cell , receptor , microbiology and biotechnology , chemotherapy , antigen , interleukin 21 , cd8
Flt‐3 ligand (FL) is a growth factor (GF) which might have clinical use as a mobilizer of stem and progenitor cells into peripheral blood (PB) in autologous transplantations of various malignant haematological diseases, unless FL stimulates the growth of malignant cells in these diseases. The present study evaluated the effects of FL on the proliferation of granulocyte‐macrophage (GM) progenitor cells collected from PB of 24 patients with chronic myeloproliferative disorders (MPDs) by using a methylcellulose assay in serum‐free culture conditions. It was shown that FL as a single factor had no stimulatory effect on GM colony formation either in the whole MPD group or in the MPD subgroups, which comprised 9 patients with essential thrombocythaemia, 7 with polycythaemia vera and 8 with chronic myelogenous leukaemia. No increase in GM colony formation was observed, either, when FL was used in combination with other GFs, such as mast cell growth factor (MGF), granulocyte‐colony stimulating factor (G‐CSF), GM‐CSF or interleukin‐3 (IL‐3). GM‐CSF and IL‐3 were the only single GFs which significantly increased GM colony formation in the whole MPD group. As a conclusion, FL does not seem to induce GM colony formation of MPDs alone or in combination with G‐CSF in in vitro colony assays.