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Ectopic haematopoiesis in the mouse: roles of stroma and cytokines in granulocytopoiesis in in vivo diffusion chamber cultures
Author(s) -
Wang Xiuli,
StrømGundersen Inger,
Fjerdingstad Hege,
Benestad Haakon B.
Publication year - 1999
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1999.tb01134.x
Subject(s) - stromal cell , haematopoiesis , bone marrow , progenitor cell , stroma , biology , immunology , in vivo , population , granulocyte , peritoneal cavity , microbiology and biotechnology , stem cell , cancer research , medicine , immunohistochemistry , anatomy , environmental health
We have examined a possible role of two different types of irradiated stromal cells, i.e. murine bone marrow (BM) stromal cells and stromal cell line MS‐5R, when cocultured with murine blood‐borne progenitors or sorted Lin − Sca‐1+ bone marrow cells in vivo in peritoneal diffusion chambers (DC). Retrieval and quantification of the cultured cells were performed after 4, 7, and 14 d. Granulocyte and/or macrophage colony‐forming cells (G/M‐CFC) were enumerated in subcultures from the DC. G/M‐CFC production was not enhanced in the stroma‐contact cultures, in comparison with the standard stroma‐non‐contact cultures, but early granulocytopoiesis was stimulated. Perturbation of the humoral environment of DC was investigated in a number of ways, for example with continuous infusion of rhG‐CSF from a subcutaneous implanted minipump to DC host mice, with DC host mice carrying a transplantable leukaemia, secreting interleukin 3 (IL‐3), and with injections of various cytokines. None of these interventions sustained the expansion of the G/M‐CFC population. In conclusion, for ectopic haematopoiesis to take place, several requirements must be met. Relevant stromal cells apparently affect haematopoiesis both via direct cell‐cell interactions and via humoral mediators (viz. cytokines) which they secrete.