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Modulatory function on autologous myeloid progenitor cells of clonal T‐lymphocytes following autologous bone marrow transplantation
Author(s) -
Hokland Peter,
Carlsen Ingrid,
Hokland Marianne,
Nielsen Bendt
Publication year - 1998
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1998.tb01021.x
Subject(s) - progenitor cell , myeloid , bone marrow , immunology , transplantation , medicine , function (biology) , bone marrow transplantation , cancer research , biology , stem cell , microbiology and biotechnology
We have studied the regulatory capacity of clonal T‐lymphocytes from patients undergoing autologous bone marrow transplantation (ABMT) on the generation of CFU–GM from their harvest marrow preparations. To this end, T‐lymphocytes from peripheral blood from 5 patients undergoing ABMT isolated 10 d before and 7, 14 and 28 d post‐ABMT were placed in limiting dilution conditions (384 wells for each patient at each time point) and polyclonally stimulated. From more than 1600 wells with growth from the 5 patients, preparations from more than 900 wells could be expanded (range between patients 33–452) and identified by immunophenotyping (IP) and flow cytometry (FCM) by their exclusive expression of CD4 or CD8. This was significantly fewer than seen in normal donors, especially so at d 7 and 14 post‐ABMT. The ratio between CD4+ and CD8+ clones varied between 0.6 and 2.8 (median 1.3) and was significantly lower in the patients compared to normal donors (median 3.1; range 3.0–6.5). When the clonal T‐cell preparations were co‐cultured with autologous bone marrow cells obtained at the time of harvest and depleted for T‐lymphocytes, the vast majority of both CD4+ and CD8+ clones exerted a clear enhancement on the CFU–GM growth with no relation to time of blood sampling in its the magnitude. Moreover, a trend seen in the normal donors towards CD4+ clones being more effective in this enhancement was not observed in ABMT patients. We conclude that clonal T‐cells from ABMT patients, irrespective of their phenotype and time of isolation, exert an enhancement on the growth of autologous CFU–GM, which is equal to that seen in normal donors.