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Different expression of adhesion molecules on myeloid and B‐lymphoid CD34 + progenitors in normal bone marrow
Author(s) -
Waele Marc,
Renmans Wim,
Damiaens Serge,
Flament Jocelyne,
Schots Rik,
Riet Ivan
Publication year - 1997
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1997.tb01687.x
Subject(s) - cd34 , myeloid , bone marrow , progenitor cell , biology , cell adhesion molecule , antigen , population , haematopoiesis , lymphopoiesis , microbiology and biotechnology , immunology , stem cell , medicine , environmental health
The expression of adhesion molecules was studied on B lymphoid and myeloid CD34 + precursors in normal bone marrow. Bone marrow aspirates were labelled in a double fluorescence procedure with the CD34 monoclonal antibody 43A1 and with antibodies directed against maturation and differentiation antigens and adhesion molecules. Three clusters of CD34 + cells could be distinguished by their light scatter characteristics in flow cytometry. The population with the lowest forward scatter contained B‐lymphoid precursors while the two others showed phenotypic characteristics of, respectively, early and late myeloid precursors. Nearly all CD34 + cells in the 3 subpopulations expressed VLA‐4, VLA‐5, LFA‐3 and H‐CAM. B‐lymphoid progenitors showed a higher density of VLA‐4 and VLA‐5 than the myeloid progenitors. Myeloid precursors, and particularly the late subset, expressed more HCAM than the B‐lymphoid progenitors. The majority of the CD34 + cells also expressed LFA‐1 and L‐selectin. Higher numbers of positive cells were found in the myeloid subset. The early myeloid subset showed the highest positivity for L‐selectin. We conclude that B lymphoid and early and late myeloid CD34 + precursors in normal bone marrow show a different profile of adhesion molecules. These profiles could reflect a higher tendency of the myeloid CD34 + precursors to circulate.