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Platelet membrane fluidity and intraplatelet Ca 2+ mobilization are affected in uraemia
Author(s) -
Walkowiak B.,
Borkowska E.,
Koziolkiewicz W.,
Michalec L.,
Sobol A.,
Cierniewski C. S.
Publication year - 1997
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1997.tb01682.x
Subject(s) - platelet , chemistry , thrombin , medicine , endocrinology , platelet activation , basal (medicine) , diabetes mellitus
Abstract: In present investigations, platelet membrane fluidity and intraplatelet Ca 2+ mobilization were analysed in uraemic platelets by fluorescence techniques. Thirteen non‐dialyzed uraemic patients and 16 control subjects were examined. Anisotropy of DPH‐probe, measured at 37°C, was significantly higher in control (0.2236 ± 0.0050) than in uraemic platelets (0.1969 ± 0.0082; p < 0.01). There was no difference between control (109.8 ± 6.0 n m ) and uraemic platelets (100.0 ± 7.3 n m ) when the basal [Ca 2+ ] i in resting platelets was determined. Activation of platelets by ADP (12.5 μ m ) or by thrombin (0.1 U/ml) resulted in an increase in [Ca 2+ ] i . It was significantly higher ( p * < 0.003 for ADP and p * < 0.009 for thrombin, respectively) in control platelets (383.6 ± 56.3 n m and 2031.0 ± 298.8 n m , respectively) than in uraemic ones (191.0 ± 21.3 n m and 838.7 ± 144.1 n m , respectively). The amount of released Ca 2+ was higher in control platelets activated by both ADP and thrombin (157.6 ± 21.4 n m and 409.3 ± 71.0 n m , respectively) than in uraemic platelets (76.7 ± 15.7 n m and 203.0 ± 29.3 n m , respectively) and the differences were significant ( p < 0.01 and p * < 0.01, respectively). These results indicate an abnormal intracellular Ca 2+ mobilization in uraemic platelets. Both increased membrane fluidity and decreased Ca 2+ mobilization should be considered as a possible reason of reduced fibrinogen receptor exposure on uraemic platelets.