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Production of recombinant human GM‐CSF‐EPO hybrid proteins: in vitro biological characterization
Author(s) -
Coscarella A.,
Carloni C.,
Liddi R.,
Mauro S.,
Novelli S.,
Mele A.,
Masella B.,
Valtieri M.,
Santis R.
Publication year - 1997
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1997.tb00983.x
Subject(s) - chinese hamster ovary cell , erythropoietin , biology , recombinant dna , microbiology and biotechnology , dihydrofolate reductase , in vitro , haematopoiesis , clonogenic assay , cell culture , transfection , colony stimulating factor , gene , biochemistry , stem cell , genetics
Selective lineage differentiation depends upon the combined action of several colony‐stimulating factors. Here we describe 3 human granulocyte‐macrophage colony‐stimulating factor‐erythropoietin (GM‐CSF‐EPO) hybrid proteins generated by recombination of the relevant cDNAs. The expression vector containing the murine cytomegalovirus (mCMV) promoter and dihydrofolate reductase (DHFR) gene was used for the expression of the hybrid genes in Chinese hamster ovary (CHO) cells. Purified hybrid proteins from CHO transfectant cultures induced proliferation of both EPO and GM‐CSF dependent cell lines. The clonogenic test, performed on purified human hematopoietic precursor cells, indicates that the hybrid proteins are more efficient at inducing erythroid differentiation compared with the equimolar mixture of GM‐CSF and EPO.