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Effects of thrombopoietin ( c‐mpl ligand) on growth of blast cells from patients with transient abnormal myelopoiesis and acute myeloblastic leukemia
Author(s) -
Hirai Hideyo,
Shimazaki Chihiro,
Yamagata Noboru,
Goto Hideo,
Inaba Tohru,
Kikuta Takehisa,
Sumikuma Toshiya,
Sudo Yoshikazu,
Ashihara Eishi,
Fujita Naohisa,
Hibi Shigeyoshi,
Imashuku Shinsaku,
Ito Etsuro,
Nakagawa Masao
Publication year - 1997
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1997.tb00957.x
Subject(s) - thrombopoietin , myelopoiesis , acute myeloblastic leukemia , stem cell factor , thrombopoiesis , haematopoiesis , cancer research , immunology , biology , platelet , leukemia , medicine , stem cell , megakaryocyte , microbiology and biotechnology
Thrombopoietin (TPO) is a ligand for c‐mpl that promotes both proliferation and differentiation of megakaryocytes in vivo and in vitro. We investigated the expression of c‐mpl transcripts and the effects of recombinant human TPO (rhTPO) on the proliferation and differentiation of human leukemic cell lines or fresh samples obtained from 32 patients with transient abnormal myelopoiesis (TAM) or acute myeloblastic leukemia (AML). Cells were cultured with TPO alone or combined with rh interleukin‐3 (IL‐3) or stem cell factor (SCF). Expression of c‐mpl was verified in 6 of 13 cases tested. All but one of the cases that showed c‐mpl expression responded to TPO. Blasts from all cases of TAM or French–American–British (FAB) subtype M7 showed growth responses to TPO with higher sensitivity than cells of other FAB subtypes and these responses were increased by addition of rhIL‐3 or rhSCF in some cases. Responses of cells of other FAB subtypes varied. In addition, increased expression of platelet‐specific surface antigens on MO7E cells after incubation with rhTPO was observed. These data suggest that TPO may be involved in the abnormal proliferation and differentiation of human leukemic cells, especially of M7 and TAM cells, considered to be of megakaryocytic lineage.