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Increased serum levels of macrophage colony‐stimulating factor (M–CSF) in α‐and β‐thalassaemia syndromes: correlation with anaemia and monocyte activation
Author(s) -
Wiener Edith,
Wanachiwanawin Wanchai,
Chinprasertsuk Sriprapa,
Siripanyaphinyo Uamporn,
Mawas Fatme,
Fucharoen Suthat,
Wickramasinghe Sunitha N.
Publication year - 1996
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1996.tb01394.x
Subject(s) - medicine , macrophage colony stimulating factor , endocrinology , basal (medicine) , monocyte , iron deficiency , anemia , macrophage , chemistry , in vitro , biochemistry , insulin
Serum levels of M–CSF were determined by an ELISA method in 29 and 34 patients with HbH disease (α 1 /α 2 or α 1 /HbCS) or β 0 ‐thal/HbE, respectively, in 28 haematologically normal subjects and in five patients with anaemia due to iron deficiency or myelodysplasia. In HbH disease and β 0 ‐thal/HbE, M–CSF concentrations were significantly higher than those in the normal subjects [986 ± 138 and 1385 ± 133, respectively, vs. 500 ± 33 pg/ml (mean ± SEM); p <0.01, and p <0.001, respectively]. By contrast, in patients with anaemia due to iron deficiency, M–CSF levels were within the normal range. In HbH disease and in β 0 ‐thal/HbE, M–CSF levels correlated inversely with mean basal Hb values ( r = –0.39, p = 0.05 and r = –0.60, p <0.001, respectively). In addition, in some of the HbH and β 0 ‐thal/HbE patients, monocyte ADCC activities towards red cells were tested and found to be approximately twice as high as those in normal controls [38.3±5.7 and 30.7 ± 4.6 vs. 17.8 ± 1.8 % specific lysis (mean ± SEM), respectively; p <0.01 and p <0.02, respectively]. When thalassaemic patients and normal controls were considered together there was a significant correlation between M–CSF levels and monocyte ADCC activities ( r = 0.51, p <0.02). The results suggest that in HbH disease and in β 0 ‐thal/HbE, raised serum M–CSF contributes to the anaemia by enhancing the effector function of mononuclear phagocytes towards red cells.