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Strong increase in the percentage of the CD8 bright+ CD28 ‐ T‐cells and delayed engraftment associated with cyclosporine‐induced autologous GVHD
Author(s) -
Garin Laure,
Rigal Dominique,
Souillet Gerard,
Bernaud Janine,
Mérieux Yves,
Philippe Noel
Publication year - 1996
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1996.tb01329.x
Subject(s) - cd8 , medicine , transplantation , immunology , cd28 , cytotoxic t cell , aplasia , t lymphocyte , lymphocyte , gastroenterology , immune system , biology , in vitro , biochemistry
Four children with acute lymphoblastic leukaemia had autologous bone marrow (BM) or peripheral stem cell (PSC) transplantation with low dose of cyclosporine (CsA, 1 mg/kg/d i.v. during the first 28 d) to induce an autologous GVHD (auto‐GVHD). Two children did not have clinical auto‐GVHD and they relapsed 3 and 4 months after treatment. The 2 other children had clinical signs of auto‐GVHD (grade I and grade II): they both are in complete remission but after a first normal haematological recovery they had a prolonged period of aplasia until month 9 for 1 patient and still persistent at month 7 in the other case. We studied lymphocyte subsets reconstitution after transplantation in these patients. All patients had an important decrease in the CD4/CD8 ratio related both to a strong decrease in the CD4 + cells and a strong increase in the CD8 + cells. Most of the CD8 + cells were of the CD8 bright+ CD28 ‐ phenotype. These CD8 bright+ CD28 ‐ T‐cells represented from 33% to 68% of the total lymphocytes. We discuss the role of these cells after autologous transplantation with CsA, and wonder if these cells could mediate cytotoxicity. In conclusion, among 4 children who received autologous BM or PBC transplantation with low dose of CsA, we observed a complete remission after an auto‐GVHD and a prolonged period of aplasia in 2 patients and a relapse of leukaemia in 2 other patients. All these 4 patients had an increase in the CD8 bright+ CD28 ‐ T lymphocytes.