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Simplification of the blood stem cell transplantation (BSCT) procedure: large volume apheresis and uncontrolled rate cryopreservation at –80°C
Author(s) -
Feremans W. W.,
Bastin G.,
Moine F. Le,
Ravoet C.,
Delville J. P.,
Pradier O.,
Wallef G.,
Dupont E.,
Capel P.,
Lambermont M.
Publication year - 1996
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1996.tb00715.x
Subject(s) - cryopreservation , apheresis , medicine , transplantation , stem cell , surgery , urology , biology , microbiology and biotechnology , platelet , embryo
Very high‐dose chemotherapy with autologous blood stem cell (BSC) rescue becomes more and more widely performed. In order to simplify the technique, a large volume apheresis programme combined with an uncontrolled rate cryopreservation at –80°C was developed. Twenty‐six patients suffering from multiple myeloma ( n = 8), non‐Hodgkin's lymphoma ( n = 7), dysgerminoma ( n = 4), breast cancer ( n = 3), Hodgkin's disease ( n = 2), acute lymphoblastic leukaemia ( n = 1) and acute myelocytic leukaemia ( n = 1) were autografted after a classical high‐dose chemotherapy regimen. A single large volume apheresis was sufficient to obtain the threshold value of CD34+ BSC in 24/26 transplantations. The haematological recovery was favourably comparable with the previously published data obtained with controlled rate frozen BSC: median time to granulocytes > 1000/μL and to a self‐supporting platelet count > 20,000/μL, respectively, 10.5 and 12 d. The treatment‐related mortality was confined to 1/26 BSCT. These results indicate that this easy and cost‐saving policy of BSCT is efficacious and safe: sustained long‐term haematopoiesis, reduced morbidity and mortality were observed.