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Changes in adhesion molecule expression and function in B‐cell chronic lymphocytic leukaemia after in vitro interferon‐α stimulation
Author(s) -
Csanaky G.,
Vass J. A.,
Ocsovszki I.,
Milosevits J.,
Szomor Á.,
Schmelczer M.
Publication year - 1995
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1111/j.1600-0609.1995.tb01622.x
Subject(s) - cell adhesion molecule , stimulation , peripheral blood mononuclear cell , selectin , in vitro , alpha (finance) , immunology , microbiology and biotechnology , biology , chemistry , medicine , endocrinology , biochemistry , construct validity , nursing , patient satisfaction
Peripheral blood mononuclear cells (PBMCs) from 10 B‐CLL patients were investigated after 24 hours of in vitro interferon‐α (IFN‐α) stimulation. The constitutional expression of the L‐selectins (LECAM‐1), LFA‐1/CD11a, VLA α‐4/CDw49d and ICAM‐1/CD54 adhesion molecules was detected, and changes in their density after IFN‐α stimulation were compared to results obtained by the high endothelial venule (HEV)‐binding assay and a carbohydrate (phosphonomannan core polysaccharide: PPME and fucoidin) immobilization test. The LECAM‐1 and ICAM‐1 molecules were expressed on the great majority of CLL cells, while the LFA‐1 and VLA‐4 α‐chains were expressed by only a small number of cells. Statistically significant changes (p< 0.001) were observed in LECAM‐1 antigen density (changes in mean cell fluorescence), as well as in functional tests (HEV‐, PPME‐ and fucoidin‐binding; p<0.01) after in vitro IFN‐α stimulation. Based on a prior study (Jewell et al., Leukemia 1992: 6: 400–404) and on the present findings, not only an increased expression but also an enhanced function of the L‐selectins seem to be well substantiated after IFN‐α stimulation, which may explain the therapeutic effect of IFN‐α in reducing the accumulation of leukaemic B cells in the blood. The remarkably high expression of ICAM‐1 in this series necessitates further studies to clarify the exact expression rate and role of this molecule.